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macs ms columns  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec macs ms columns
    Macs Ms Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 2422 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macs+ms+columns/MS+Columns/pm42421121-114-20-23
    Average 97 stars, based on 2422 article reviews
    macs ms columns - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    FACS:

    Article Title: Macrophage extracellular traps promote maladaptive cardiac remodelling and heart failure via PAD4-dependent mechanisms
    Article Snippet: .. Magnetic-activated cell sorting was performed on bone marrow cells using MACS MS columns (Miltenyi Biotec GmbH) with CD11b MicroBeads (130-097-142, Miltenyi Biotec GmbH) according to the manufacturer’s instructions. .. Cells were fixed with 4% paraformaldehyde, permeabilized with either 0.05% or 0.2% Triton X-100 for 3 or 10 min, and subsequently blocked with 2% bovine serum albumin for 1 h at room temperature and incubated with specific primary antibodies: anti-citrullinated histone H3 (ab5103, Abcam), CD68 (MCA1957, Bio-Rad), followed by appropriate secondary antibodies such as Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (A21245, Thermo Fisher Scientific), Anti-rat IgG (H+L), Alexa Fluor 488 (4416, Cell Signaling Technology) and then mounted with DAPI.

    Magnetic Cell Separation:

    Article Title: Macrophage extracellular traps promote maladaptive cardiac remodelling and heart failure via PAD4-dependent mechanisms
    Article Snippet: .. Magnetic-activated cell sorting was performed on bone marrow cells using MACS MS columns (Miltenyi Biotec GmbH) with CD11b MicroBeads (130-097-142, Miltenyi Biotec GmbH) according to the manufacturer’s instructions. .. Cells were fixed with 4% paraformaldehyde, permeabilized with either 0.05% or 0.2% Triton X-100 for 3 or 10 min, and subsequently blocked with 2% bovine serum albumin for 1 h at room temperature and incubated with specific primary antibodies: anti-citrullinated histone H3 (ab5103, Abcam), CD68 (MCA1957, Bio-Rad), followed by appropriate secondary antibodies such as Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (A21245, Thermo Fisher Scientific), Anti-rat IgG (H+L), Alexa Fluor 488 (4416, Cell Signaling Technology) and then mounted with DAPI.

    Article Title: Free Fatty Acids Induce Endoplasmic Reticulum Stress-Mediated Apoptosis of Macrophages in Dairy Cows with Ketosis
    Article Snippet: The bovine anti-CD14 monoclonal antibody (MCA2678GA, Bio-Rad, Hercules, CA, USA) was used to incubate the cells at 4 °C for 30 min, followed by centrifugating at 250× g for 5 min. Next, cells were incubated with MicroBeads (130-407-101, Miltenyi Biotec, Bergisch Gladbach, Germany) at 4 °C for 15 min. .. The phosphate-buffered solution (PBS) supplemented with 2.0 mM ethylene diamine tetraacetic acid (EDTA) and 5 g/L bovine serum albumin (BSA) was used to resuspend the cells, followed by isolating cells with MACS MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated CD14 + monocytes were cultured in RPMI-1640 medium (RNBJ7889, Sigma-Aldrich, St. Louis, MO, USA) containing growth-promoting molecules (0.05% α-lactose, 0.05% whey protein and 1 mg/L progesterone), 10% fetal bovine serum (FBS, FB15015, Clark Bioscience, Richmond, VA, USA), 20 ng/mL of bovine granulocyte-macrophage colony-stimulating factor (ab209168, Abcam, Cambridge, MA, USA), 10 μg/L insulin RNBJ7889 (Sigma-Aldrich, St. Louis, MO, USA), 1% penicillin (P1400, Solarbio, Beijing, China) and 20 ng/mL bovine GM-CSF (ab209168, Abcam, Cambridge, MA, USA) for 7 d to differentiate into macrophages.

    Article Title: Organ-resolved endothelial regulatory programs link aging and metabolic overload to vascular immune remodeling
    Article Snippet: For CD11b+ cell isolation, an anti-CD11b antibody (BioLegend, clone M1/70; 5 μL per sample) was added and incubated on ice for 15 min, followed by washing and incubation with SAV beads (BioLegend; 5 μL) for an additional 15 min on ice. .. After dilution to 500 μL with buffer, CD11b+ cells were isolated using MACS MS columns (Miltenyi Biotec). .. Cells were plated at 1.0 × 106 cells per well in 6-well plates and cultured in 1.5 ml of differentiation medium (DMEM high glucose, 10% FBS, 1× GlutaMAX, 1% penicillin/streptomycin) supplemented with 50 ng ml−1 M-CSF (PeproTech, AF-315-02) and 20 ng ml−1 mouse IL-4 (R&D Systems, 404-ML).

    Article Title: Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damage.
    Article Snippet: .. Bone marrow cells were obtained by flushing femurs and tibiae with PBS + 2% fetal bovine serum, followed by red blood cell lysis with ACK buffer (Lonza #10-548E).13 Neutrophils were enriched using MACS MS columns (Miltenyi #130-097-658) and stained with antibodies for Ly6G (BioLegend #127654 or #563978), CD11b (BioLegend #101259 or Thermo Fisher #12-0112-82), and viability dye (Thermo Fisher #L10120).13 Healthy human blood samples were purchased and provided to us in a de-identified manner from Hoxworth Blood Center. .. Human neutrophils were isolated using the Direct Human Neutrophil Isolation Kit (StemCell Technologies #19666) and then stained for CD11b (BioLegend #301403) and CD66b (BioLegend #392903).

    Article Title: Plant-derived mitochondria mitigate aging-related neurodegeneration by reprogramming microglial mitochondrial energy metabolism.
    Article Snippet: .. Microglia were isolated using Microglia MicroBeads (Miltenyi Biotec, Cat# 130-093- 634), and then separated using autoMACS Pro Separator with a MACS MS Columns (Miltenyi Biotec, Cat# 130-042-201). ..

    Article Title: Knockin expression of human ADAMTS5 impairs cardiovascular development and aggravates cerebral cavernous malformations in mice
    Article Snippet: The single-cell suspension was incubated with anti-CD31-conjugated magnetic beads (Miltenyi Biotec, 130-097-418) at 4°C for 15 min after they were passed through a 70 μm strainer. .. We collected microbead-bound cells using MACS MS columns (MiltenyiBiotec) for subsequent qPCR analysis. ..

    Article Title: Free Fatty Acids Induce Endoplasmic Reticulum Stress-Mediated Apoptosis of Macrophages in Dairy Cows with Ketosis.
    Article Snippet: The bovine anti-CD14 monoclonal antibody (MCA2678GA, Bio-Rad, Hercules, CA, USA) was used to incubate the cells at 4 ◦C for 30 min, followed by centrifugating at 250× g for 5 min. Next, cells were incubated with MicroBeads (130-407-101, Miltenyi Biotec, Bergisch Gladbach, Germany) at 4 ◦C for 15 min. .. The phosphate-buffered solution (PBS) supplemented with 2.0 mM ethylene diamine tetraacetic acid (EDTA) and 5 g/L bovine serum albumin (BSA) was used to resuspend the cells, followed by isolating cells with MACS MS columns (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The isolated CD14+ monocytes were cultured in RPMI-1640 medium (RNBJ7889, Sigma-Aldrich, St. Louis, MO, USA) containing growth-promoting molecules (0.05% α-lactose, 0.05% whey protein and 1 mg/L progesterone), 10% fetal bovine serum (FBS, FB15015, Clark Bioscience, Richmond, VA, USA), 20 ng/mL of bovine granulocyte-macrophage colony-stimulating factor (ab209168, Abcam, Cambridge, MA, USA), 10 μg/L insulin RNBJ7889 (Sigma-Aldrich, St. Louis, MO, USA), 1% penicillin (P1400, Solarbio, Beijing, China) and 20 ng/mL bovine GMCSF (ab209168, Abcam, Cambridge, MA, USA) for 7 d to differentiate into macrophages.

    Article Title: Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damage
    Article Snippet: Bone marrow cells were obtained by flushing femurs and tibiae with PBS + 2% fetal bovine serum, followed by red blood cell lysis with ACK buffer (Lonza #10-548E). .. Neutrophils were enriched using MACS MS columns (Miltenyi #130-097-658) and stained with antibodies for Ly6G (BioLegend #127654 or #563978), CD11b (BioLegend #101259 or Thermo Fisher #12-0112-82), and viability dye (Thermo Fisher # L10120 ). ..

    Isolation:

    Article Title: Organ-resolved endothelial regulatory programs link aging and metabolic overload to vascular immune remodeling
    Article Snippet: For CD11b+ cell isolation, an anti-CD11b antibody (BioLegend, clone M1/70; 5 μL per sample) was added and incubated on ice for 15 min, followed by washing and incubation with SAV beads (BioLegend; 5 μL) for an additional 15 min on ice. .. After dilution to 500 μL with buffer, CD11b+ cells were isolated using MACS MS columns (Miltenyi Biotec). .. Cells were plated at 1.0 × 106 cells per well in 6-well plates and cultured in 1.5 ml of differentiation medium (DMEM high glucose, 10% FBS, 1× GlutaMAX, 1% penicillin/streptomycin) supplemented with 50 ng ml−1 M-CSF (PeproTech, AF-315-02) and 20 ng ml−1 mouse IL-4 (R&D Systems, 404-ML).

    Article Title: Plant-derived mitochondria mitigate aging-related neurodegeneration by reprogramming microglial mitochondrial energy metabolism.
    Article Snippet: .. Microglia were isolated using Microglia MicroBeads (Miltenyi Biotec, Cat# 130-093- 634), and then separated using autoMACS Pro Separator with a MACS MS Columns (Miltenyi Biotec, Cat# 130-042-201). ..

    Red Blood Cell Lysis:

    Article Title: Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damage.
    Article Snippet: .. Bone marrow cells were obtained by flushing femurs and tibiae with PBS + 2% fetal bovine serum, followed by red blood cell lysis with ACK buffer (Lonza #10-548E).13 Neutrophils were enriched using MACS MS columns (Miltenyi #130-097-658) and stained with antibodies for Ly6G (BioLegend #127654 or #563978), CD11b (BioLegend #101259 or Thermo Fisher #12-0112-82), and viability dye (Thermo Fisher #L10120).13 Healthy human blood samples were purchased and provided to us in a de-identified manner from Hoxworth Blood Center. .. Human neutrophils were isolated using the Direct Human Neutrophil Isolation Kit (StemCell Technologies #19666) and then stained for CD11b (BioLegend #301403) and CD66b (BioLegend #392903).

    Staining:

    Article Title: Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damage.
    Article Snippet: .. Bone marrow cells were obtained by flushing femurs and tibiae with PBS + 2% fetal bovine serum, followed by red blood cell lysis with ACK buffer (Lonza #10-548E).13 Neutrophils were enriched using MACS MS columns (Miltenyi #130-097-658) and stained with antibodies for Ly6G (BioLegend #127654 or #563978), CD11b (BioLegend #101259 or Thermo Fisher #12-0112-82), and viability dye (Thermo Fisher #L10120).13 Healthy human blood samples were purchased and provided to us in a de-identified manner from Hoxworth Blood Center. .. Human neutrophils were isolated using the Direct Human Neutrophil Isolation Kit (StemCell Technologies #19666) and then stained for CD11b (BioLegend #301403) and CD66b (BioLegend #392903).

    Article Title: Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damage
    Article Snippet: Bone marrow cells were obtained by flushing femurs and tibiae with PBS + 2% fetal bovine serum, followed by red blood cell lysis with ACK buffer (Lonza #10-548E). .. Neutrophils were enriched using MACS MS columns (Miltenyi #130-097-658) and stained with antibodies for Ly6G (BioLegend #127654 or #563978), CD11b (BioLegend #101259 or Thermo Fisher #12-0112-82), and viability dye (Thermo Fisher # L10120 ). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Knockin expression of human ADAMTS5 impairs cardiovascular development and aggravates cerebral cavernous malformations in mice
    Article Snippet: The single-cell suspension was incubated with anti-CD31-conjugated magnetic beads (Miltenyi Biotec, 130-097-418) at 4°C for 15 min after they were passed through a 70 μm strainer. .. We collected microbead-bound cells using MACS MS columns (MiltenyiBiotec) for subsequent qPCR analysis. ..



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